Research Fellow (CRISPR Genome Editing & Off-Target Profiling)

Company description

The National University of Singapore is the national research university of Singapore.

Founded in 1905 as the Straits Settlements and the Federated Malay States Government Medical School, NUS is the oldest higher education institution in Singapore

Job Description

We are looking for a talented and motivated postdoctoral fellow to join the Genome Re-InnovaTion Lab ( part of the Synthetic Biology Translational Research Programme at the National University of Singapore, Yong Loo Lin School of Medicine.

This position is funded by a Ministry of Education Tier-2 grant focused on improving the safety of genome editing tools through high-throughput experimental profiling and artificial intelligence.

The successful candidate will lead efforts to generate and analyze genome-wide data that capture how editing enzymes interact with DNA in various chromatin and cellular contexts.

This work will directly support the development of AI models to predict off-target effects across clinically relevant cell types, including primary cells and 3D organoid systems.

Responsibilities

1. Design and perform genome editing experiments in a range of human cell types, including immortalized lines, primary cells, and organoid cultures.
   
   
2. Develop and optimize protocols to capture DNA cleavage events at high resolution using sequencing-based assays.
   
   
3. Conduct quality control, library preparation, and sample tracking for large-scale sequencing workflows.
   
   
4. Coordinate experiments involving genome editors such as Cas9, base editors, and prime editors.
   
   
5. Supervise and train junior team members including research assistants and students.
   
   
6. Work collaboratively with computational researchers to integrate experimental data into predictive models.
   
   
7. Contribute to manuscript preparation, presentations, and funding reports.
   
   

Qualifications

1. PhD in Molecular Biology, Biochemistry, Biotechnology, or a related life sciences field.
   
   
2. Extensive hands-on experience with genome editing technologies and mammalian cell culture.
   
   
3. Strong skills in molecular cloning, nucleic acid extraction, PCR/qPCR, and next-generation sequencing workflows.
   
   
4. Prior experience with functional genomic assays or high-throughput screening is advantageous.
   
   
5. Familiarity with working on complex cell models such as primary cells or 3D cultures is a plus.
   
   
6. Ability to troubleshoot independently, manage multiple projects, and work well in collaborative environments.
   
   
7. Strong publication record in peer-reviewed journals.
   
   

Remuneration will be commensurate to the candidate's qualifications and experience.

Informal enquiries are welcome and should be made to

Ms Suhana Sulaiman , at

Formal application

Please submit your application, indicating job reference number and current/expected salary, supported by a detailed CV (including personal particulars, academic and employment history, complete list of publications/oral presentations and full contacts of 3 referees).

Only shortlisted candidates will be notified.

More Information

Location: Kent Ridge Campus

Organization: Yong Loo Lin School of Medicine

Department : Dean-Med-Synthetic Biology Translational Research Programme

Employee Referral Eligible: No

Job requisition ID : 28587